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Immediately before heat treatments or prior to AGEs analysis, the white muscle above the lateral line on both sides of a fish steak was carefully removed, cut into small cubes and ground at low speed for 20 s and then high speed for 10 s in a homogenizer (8010 s; Waring, Inc., Torrington, CT).
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A Grindomix GM 200 knife mill (Retsch Inc., Haan, Germany) was used to grind (5000 rpm, 20 seconds) FBS to particle sizes of about 500 μm, while a homogenizer (8010 s, Waring, Inc., Torrington, Connecticut, USA) was used (at high speed for 20 seconds) to obtain particle sizes ranging from about 1000 to 2000 μm. The ground FBS particles were ...
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